FAQs

Why Artennua® and not another product?

What is Artennua® and what differentiates us from others?

Artennua® is the optimized version of the Artemisia annua plant. The Artemisia annua plant (or also qinghao in Chinese) is the scientific name of the medicinal plant of the Asteraceae family, used for 2000 years in traditional Chinese medicine, thanks to its antipyretic effect.

Artennua® is its enhanced version thanks to our 100% natural technology that increases the content of active botanical molecules. The production of Artennua® is based on a unique and patented technology called TricoStimulant™, a 100% natural and intelligent agrotechnological solution, in the form of a biostimulant specifically aimed at improving medicinal and aromatic plants. The mechanism of action behind TricoStimulant™ technology is based on stimulating the trichomes (glandular structures) of plants to produce more molecules of interest. After many years of R&D, currently, our largest production originates from an organic farm in the Canary Islands (Spain).

Artennua® is a pure ingredient that is not derived from any type of extraction process and differs from other commercially available extracts by increasing at least x2 the density of the trichomes, as well as x2 the concentration of the main therapeutic metabolites of the plant (artemisinin, eupatorin and casticin). Compared to other commercial Artemisia annua raw materials available on the market, as well as 30:1 or 10:1 extracts, Artennua® shows a higher artemisinin content.

What is the difference with dry extracts of Artemisia annua?

Dry extracts are the most common commercial format of the A. annua plant available on the market. They are the result of a concentration process of the plant, which concentrates artemisinin and some molecules, but loses many others, which are important both for their therapeutic value and for increasing the bioavailability of artemisinin (for its absorption into the blood). Extracts often have efficacy problems, precisely due to this lack of absorption of artemisinin into the blood.

Why does Artennua® only use plant leaves?

Artennua® only uses the leaves of A. annua because that is where the glandular trichomes of the plant are found and, therefore, where most of the active ingredients are produced and stored. The rest of the plant has very little content of artemisinin and other therapeutically valuable molecules. Using the whole plant increases the amount of raw material, but decreases the content of active ingredients.

Pharmacodynamic synergy between various therapeutic biomolecules present in Artennua® leaves.

In the A. annua plant there is not only artemisinin as a medical compound, but also 200 other molecules such as arteannuin B, casticin, 1-8 cineole (eucalyptol), scopoletin, quercetin and many more. These molecules have a therapeutic value that has been confirmed by scientific publications and articles. The presence of these molecules or secondary metabolites, in addition to improving and increasing the effectiveness of artemisinin (in science it is known as pharmacodynamic synergy), also improves its bioavailability, that is the ability of a molecule to be absorbed into the bloodstream. Thanks to the presence of these molecules, the bioavailability of artemisinin is 40 times greater than that of isolated artemisinin (other commercial extracts) and in Artennua® we significantly increase not only artemisinin but also some of these molecules. Desrosiers MR, Mittelman A, & Weathers PJ., 2020

Formats and compositions

Composition of Artennua® capsules

Artennua® is made from the powdered leaves of the optimized version of Artemisia annua, which means that it is a 100% natural product without additives or flavorings. Its composition is 200 mg Artennua® powder + 180 mg rice protein + 95 mg “microcrystalline cellulose” flow agent of plant origin. Therefore, we have 1.3 mg of artemisinin in 100 mg; i.e. 2.6 mg of artemisinin in one capsule.

Composition of Artennua® “tablets”

The composition of the Artennua® tablet is 950 mg of A. annua powder (1.1% artemisinin) + 200 mg of rice protein + 150 mg of dicalcium phosphate + 700 mg of caking agent “microcrystalline cellulose” (plant origin).

Mode of use and dosage

Method of use and administration

As a complement to other treatments: 2 months of uninterrupted treatment, followed by veterinary control of clinical status and antibody levels. In a long-term maintenance regimen, it is advisable to take breaks of at least 1 week in between to improve effectiveness (artemisinin receptors can become saturated for a prolonged period). For prevention: 1 month of treatment and 3 months of rest. This cycle can be repeated up to 3 times in the same year. It is advisable to always follow the guidelines provided by the veterinarian on a case-by-case basis.

Always follow the guidelines provided by your veterinarian.

Dosage

Capsule dosage information:

0-10 kg: 1 capsule/day (200 mg A. annua, 2.6 mg Artemisinin) 11-20 kg: 2 capsules/day (400 mg A. annua, 5.2 mg Artemisinin) 21-30 kg: 3 capsules/day (600 mg A. annua, 7.8 mg Artemisinin) 31-40 kg: 4 capsules/day (800 mg A. annua, 10.4 mg Artemisinin) 41-50 kg: 5 capsules/day (1000 mg A. annua, 13 mg Artemisinin)

Tablet dosage information:

40 kg: 1 tablet/day (950 mg of A. annua powder, 10.45 mg Artemisinin)

Should the capsules be given whole or can the contents be sprinkled on food?

The experience we have had from veterinarians with the capsules is good palatability. However, if a dog finds it difficult to take the capsules, they can be opened and mixed with food.

Are Artennua® tablets divisible?

The tablets can be divided into 2 (for 20 kg dogs) or 4 (for 10 kg dogs).

10 kg: 0.25 tablet/day (divide 1 tablet into 4 equal parts and administer only one part) (237.5 mg of A. annua powder, 2.61 mg Artemisinin)

20 kg: 0.5 tablet/day (divide 1 tablet into 2 equal parts and administer only one part) (475 mg of A. annua powder, 5.22 mg Artemisinin)

Scientific evidence: Our own studies with Artennua®

1) Leishmaniosis

Promastigotes (parasite at the beginning of its life cycle)

An in vitro study was carried out to analyze the leishmanicidal effect of Artennua® and compare it with another commercial product based on A. annua extract (30:1) but with a much lower concentration of artemisinin. The biological activity against promastigotes of Leishmania infantum was studied; and the results show that Artennua® at concentrations higher than 1.2% artemisinin is 7.9 times more effective than the commercial product chosen based on A. annua. Showing how said effectiveness is directly proportional to the concentration of artemisinin.

Subsequently, another in vitro study was carried out where murine macrophages (J774A.1) were infected with Leishmania infantum amastigotes. The results show the leishmanicidal activity of Artennua® by clearly reducing the parasite load of said amastigotes.

2) Immune system

a) IL 6 with ideal/neutral value (without inflammation)

b) IL 6 with very high value (inflamed)

IL6 is a marker of the immune system. When there is an external agent attacking our body, the concentration of IL6 increases significantly. We carried out different in vitro tests where in the first case there was no inflammation and in the next case inflammation was induced.

– Without inflammation process:

Artennua® extract at 35% (v/v) significantly reduces the secretion of IL-6 in Raw264.7 macrophages, a marker of the immune system. Therefore, a preventive effect could be attributed to it.

– With inflammation process:

Artennua® extract at 35% (v/v) significantly reduces IL-6 secretion in Raw264.7 macrophages when stimulated with LPS to induce an inflammatory process. Hydrocortisone was used as a positive control.

Similar results were obtained when we performed similar in vitro assays with IL-8 and TNF-alpha.

3) Antioxidant capacity and biocidal properties.

In addition to the active compound artemisinin, recent studies show that A. annua is one of the four medicinal plants with the highest level of oxygen radical absorption capacity (ORAC). The ORAC value, or “ORAC score,” is a method for measuring the in vitro antioxidant capacity of different foods and supplements. Artemisia annua has the ability to produce high phenolic compounds, resulting in high antioxidant activity.

Artennua® extract has a much higher total antioxidant capacity (447 µmol Trolox/g) than stevia leaves (31 µmol Trolox/g), rosemary (112 µmol Trolox/g), lavender (226 µmol Trolox/g) and basil (355 µmol Trolox/g) being lower than oregano (766 µmol Trolox/g) and mint (645 µmol Trolox/g). Furthermore, in recent years various in vitro tests carried out internally and in collaboration with research centers have demonstrated the biocidal activity of Artennua® against various pathogens, both for animal health and for the food industry (Escherichia coli, Listeria sp., Candida albicans, Staphylococcus aureus) as well as for phytopathogens (Fusarium, Botrytis and Alternaria).

Other FAQs

Is the use of Artennua® compatible with other treatments?

Our product is compatible with conventional treatments such as Glucantime, Allopurinol and Miltefosine for leishmaniosis.

It is important to note that our product must always be administered under veterinary supervision and can be used as a complement to other treatments for leishmaniasis and subsequently as immune maintenance.

Is everything suitable for allergic pets?

The only allergenic substance identified in the A. annua plant is found in the pollen, but we are harvesting the material before it flowers, so no flowers are produced at harvest time; Therefore, the material is free of pollen. So far we have not had any allergy related issues with any vet and/or client. In addition, we have carried out cytotoxicity studies in macrophages with Artennua®, demonstrating non-cytotoxicity at different concentrations.

The product is 100% natural, without additives or flavorings.

Can we administer this product to cats?

The Artemisia annua plant has multiple scientifically proven benefits for animal health and can also be used in cats.